Short communication Effect of prenatal manganese intoxication on [H]glucose uptake in the brain of rats lesioned as neonates with 6-hydroxydopamine

نویسنده

  • Adam Kwieciñski
چکیده

In the present study we examined the effects of prenatal manganese (Mn) intoxication on [ H]glucose uptake in the brain of rats lesioned as neonates with 6-hydroxydopamine (6-OHDA). MnCl • 4H O (10,000 ppm) was added to the drinking water of pregnant Wistar rats for the duration of pregnancy. On the day of parturition, Mn was discontinued as an additive to the drinking water. The control group consisted of rats that consumed water without Mn. Three days after birth, rats in both groups (control and Mn) were pretreated with desipramine hydrochloride (20 mg/kg) and pargyline hydrochloride (50 mg/kg) and injected bilaterally icv with one of three doses of 6-OHDA hydrobromide (15 μg, 30 μg or 67 μg base form in saline on each side) or with saline (control). 6-[ H]-Dglucose (500 μCi/kg, ip) was administered to male offspring in adulthood; after 15 min, brain specimens were taken (frontal cortex, hippocampus, striatum, thalamus with hypothalamus, pons and cerebellum) for determination of radioactivity in a liquid scintillation counter. Low dose 6-OHDA (15 μg icv) increased [ H]glucose uptake in all brain regions (p < 0.05) in both control and Mnintoxicated animals. In rats lesioned with a moderate dose of 6-OHDA (30 μg icv), [ H]glucose uptake was unaltered in both control and Mn-exposed rats. High dose 6-OHDA (67 μg icv) reduced [ H]glucose uptake in all brain regions of Mn-exposed rats (except for cerebellum) compared with the saline group (all, p < 0.05). There was no change in regional brain uptake of [ H]glucose in control rats. In conclusion, this study shows that mild neuronal insult (15 μg icv 6-OHDA) increased glucose uptake in the brain while severe damage (concomitant 60 μg icv 6-OHDA and Mn treatment) significantly diminished this process.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effect of prenatal manganese intoxication on [(3)H]glucose uptake in the brain of rats lesioned as neonates with 6-hydroxydopamine.

In the present study we examined the effects of prenatal manganese (Mn) intoxication on [(3)H]glucose uptake in the brain of rats lesioned as neonates with 6-hydroxydopamine (6-OHDA). MnCl(2) . 4H(2)O (10,000 ppm) was added to the drinking water of pregnant Wistar rats for the duration of pregnancy. On the day of parturition, Mn was discontinued as an additive to the drinking water. The control...

متن کامل

Preconditioning Effect of Aerobic Exercise with Vitamin D3 Intake on VEGF Levels in 6-OHDA-Lesioned Rat Model of Parkinson's Disease

Background and Objective: The purpose of this study was to investigate the preconditioning effect of aerobic exercise with vitamin D3 consumption on vascular endothelial growth factor (VEGF) level in the 6-hydroxydopamine (6-OHDA)-lesioned rat model of Parkinson’s disease. Materials and Methods: Forty-eight male rats weighing 250-300 g were randomly assigned to 6 groups: healthy control, Parkin...

متن کامل

Malva sylvestris aqueous extract could ameliorate 6-hydroxydopamine-induced motor asymmetry with no protective effect on dopaminergic nigrostriatal neurons in the rat

Background and Objective: Parkinson’s disease (PD) is a common neurological disorder due to degeneration of dopaminergic neurons within pars compacta of substantia nigra (SNC). With regard to protective effect of Malva sylvestris (MS), this study was conducted to evaluate the effect of aquaeous extract of this plant in an experimental model of PD induced by 6-hydroxydopamine (6-OHDA). Material...

متن کامل

Ellagic Acid Protects the Brain Against 6-Hydroxydopamine Induced Neuroinflammation in a Rat Model of Parkinson’s Disease

Introduction: Neuroinflammation may play as an important risk factor in progressive degeneration of dopaminergic cells. Antioxidants have protective effects against free radicalsinduced neural damage in Parkinson’s disease (PD). In the present study, we examined the effects of ellagic acid (EA) on locomotion and neuroinflammatory biomarkers in a rat model of PD induced by 6-hydroxidopamin...

متن کامل

Neuroprotective Effect of Oral Administration of Creatine against 6-Hydroxydopamine Toxicity in Experimental Model of Parkinson's Disease

Background & Aims: With regard to the neuroprotective effect of creatine in some neurological disorders like cerebral ischemia, this study was conducted to evaluate the effect of creatine in an experimental model of Parkinson’s disease (PD). Involvement of oxidative stress was also assessed. Methods: In this experimental study, male rats (n = 40) were divided into 5 groups, i.e. sham-operated (...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2009